Abstract: The study area is located in the southern part of the Central Anatolia Neogene Basin (north of Karapınar Town andAcı Göl, Konya). The İnsuyu Limestone Unit, which has a total thickness of 100-400 m, is composed of claystone,sandstone, conglomerate and chert. There are also andezite and pyroclastic interclations within İnsuyu limestone.The claystone layers of this unit are white to pink in color and contain abundant Ostracoda and a few fresh waterGastropoda. In this study, 160 samples from 8 stratigraphic sections were collected but only samples havinglimestone lithology contained ostracods. A total of 16 ostracods taxa (11 previously known and five belonging toopen nomenclature) are systematically described asCyprideis pannonica, C. torosa, Ilyocypris gibba, I. bradyi,Candona neglecta, C. candida, C. altoides, C. decimai, Pseudocandona compressa, Heterocypris salina,Zonocypris membranea, Ilyocypris sp.1 sp.2, sp. and sp.Based on this Ostracoda fauna, the age of this unit is assigned as Pliocene-Early Pleistocene. This fauna indicates afew brackish but dominantly fresh water conditions in a lacustrine environment.